EFFECT OF SEMAX ON CARBOXYPEPTIDASE E ACTIVITY IN LIMBIC STRUCTURES OF THE BRAIN AT THE DEVELOPMENT OF A CONDITIONED FOOD-PROCURING REFLEX IN RATS

Latynova Irina Vladimirovna, Postrgraduate student, Penza State University (40 Krasnaya street, Penza, Russia), latynovai@mail.ru
Gengin Mikhail Trofimovich, Doctor of biological sciences, professor, head of sub-department of biochemistry, Penza State University (40 Krasnaya street, Penza, Russia), gengin07@yandex.ru
Sollertinskaya Tat'yana Nikolaevna, Doctor of medical sciences, professor, leading researcher, Institute of Developmental Physiology and Biochemistry named after I. M. Sechenov of the Russian Academy of Sciences (144 Maurica Thorez avenue, Saint-Petersburg, Russia), tns-peptidus@mail.ru
Solov'ev Vladimir Borisovich, Doctor of biological sciences, associate professor, sub-department of biochemistry, Penza State University (40 Krasnaya street, Penza, Russia), solowew@rambler.ru
Zhivaeva Lyubov' Vladimirovna, Postgraduate student, Penza State University (40 Krasnaya street, Penza, Russia), lyubasha8891@mail.ru

577.151.6

Background. The article aims at studying the effect of semax on carboxypeptidase E activity in the hippocampus and amygdala at the development of a conditioned food-procuring reflex in rats.
Materials and methods. The experiment was carried out in white nonpedigreed rats of 200-250 gramms in mass in the laboratory of the sub-department of biochemistry at Penza State University. In the first part of the experiment the authors studied the effect of the semax on the conditioned food-procuring reflex formation. In the second part, the researchers studied the effect of the semax on carboxypeptidase E activity at the development of the conditioned food-procuring reflex.
Results. The synthetic analogue of the adrenocorticotropic hormone (ACTH 4-7) the heptapeptide semax has a nootropic effect; it enhances the selective effect on the perception of information, improves memory consolidation, increases ability to training, enhances the adaptability of the brain. The effect of semax on activity of carboxypeptidase E in the hippocampus and amygdala at the development of the conditioned food-procuring reflex in rats was studied. This enzyme detaches the arginine and lysine from the C-terminus of molecules-precursors of biologically active peptides. It is shown that the semax causes significant changes of the activity of carboxypeptidase E in the limbic structures of the brain in learning animals. The results demonstrate the stimulating effect of semax on the activity of the peptidergic system. It is assumed that such a change can be one of the mechanisms of action of the studied drug, which is mediated through its nootropic effect.

semax, hippocampus, amygdala, carboxypeptidase E.

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